P6 GAG protein

HIV p6 mediates interactions between p55 and Vpr, leading to the incorporation of Vpr into assembling virions.




STRUCTURE NOT AVAILABLE
[animated gif: 320x240 ]
ViralZone:HIV-1
PDB: not available
SwissProt: P04591 (HIV-1 HXB2 GAG)
Chime Tutorial: not available
Los Alamos HIV structure DB: not available
EMBL: K03455 [EMBL/GenBank/DDBJ]
BioAfrica: Gag Protein Data Mining Tool


Isoforms:

  • p6 (52 amino acids)

Cleavage site:

Localization:

  • Virion
  • Cell cytoplasm

Function:

  • Mediates interactions between p55 and Vpr (Ref. #4)
  • Initiates incorporation of Vpr into assembling virions
    (Ref. #4)

Additional Information:

  • contains a late domain, which is necessary for the efficient release of budding virions from an infected cell

Genomic Location: [TOP]

Reference Sequences:

HIV-1 (HXB2):

          10         20         30         40         50 
| | | | |
LQSRPEPTAP PEESFRSGVE TTTPPQKQEP IDKELYPLTS LRSLFGNDPS SQ

[download in fasta format]

Length: 52 amino acids
Molecular Weight: 5784 Da
Theoretical pI: 4.60


Protein Domains/Folds/Motifs: [TOP]




Secondary Structure prediction:

Antigenic Sites - EMBOSS:

Predicted Motifs: Printer-friendly version

N-glycosylation:
N-myristoylation:
Amidation:
Protein kinase C:
Casein kinase II:
Tyrosine kinase:
cAMP / cGMP kinase:
Cell attachment motif:
Asp Protease motif:
Asp Prot Retro motif:
Cysteine-rich Region:
Tryptophan-rich Region:
Zinc-finger CCHC motif:
Leucine Zipper motif:

Protein-Protein Interactions: [TOP]

  • HIV p6 interacts with the HIV Gag (p55) polyprotein
  • HIV p6 interacts with HIV Vpr
  • HIV p6 interacts with proteins involved in the human multivesicular bodies (MVB) biogenesis pathway facilitating budding of virions
  • Some of the proteins involved in the MVB pathway include: AIP1, TSG101/ESCRT-I and CHMP4/ESCRT-III


Primary and Secondary Database Entries: [TOP]

Identifiers:

ViralZone: HIV-1
PDB/MMDB: Search for HIV & p6

SwissProt: P04591 (HIV-1 HXB2 GAG)
EMBL: K03455; AAB50258.1 [EMBL/GenBank/DDBJ]

PIR: UNKNOWN
HIV: K03455; UNKNOWN$HXB2
InterPro: none
Pfam: PF00558UNKNOWN
Prints: none
ProDom: PD454601 (1 - 52) / PD149817 (1 - 38) / PD425744 (2 - 52) / PD613638 (2 - 49)
SCOP: none
BLOCKS: P04591
Prosite: P04591
ProtoNet: P04591
ProtoMap: P04591
PRESAGE: P04591
Database of Interacting Proteins: P04591
ModBase: P04591
Swiss-2DPAGE: 2D gel

BioAfrica Tools:
- Gag Protein Data Mining Tool provides real-time analysis of HIV-1 Gag isolates
- HIV Structure BLAST searches for similar HIV sequences that have known structures
- HIV Proteomics Resource contains protein sequence and structure analysis tools

Reviews and References: [TOP]

Cite the resource by citing the following paper:
Doherty R et al. BioAfrica's HIV-1 Proteomics Resource: Combining protein data with bioinformatics tools. Retrovirology (2005), 9;2(1):18.

1 - HIV Sequence Compendium 2000
Kuiken CL, Foley B, Hahn B, Korber B, Marx PA, McCutchan F, Mellors JW, Mullins JI, Sodroski J, Wolinksy S.
Theoretical Biol. & Biophys. Group, Los Alamos Nat Lab, LA-UR 01-3860 [Read it online: Compendium]
2 - Retroviruses
Coffin JM, Hughes SH, Varmus HE.
CD-ROM ed. (2002) Cold Spring Harbor Laboratory Press [Read it online: NCBI Bookshelf]
3 - Molecular Characteristics of HIV-1 Subtype C Viruses from KwaZulu-Natal, South Africa:
Implications for Vaccine and Antiretroviral Control Strategies.
Gordon M, De Oliveira T, Bishop K, Coovadia HM, Madurai L, Engelbrecht S, Janse van Rensburg E, Mosam A, Smith A, Cassol S.
Journal of Virology 77(4): 2587-2599 (2003) [pubmed: 12551997]
4 - Incorporation of Vpr into human immunodeficiency virus type 1 virions: Requirement for
the p6 region of gag and mutational analysis.
Paxton W, Connor RI, Landau NR.
J Virol 67: 7229-7237 (1993) [pubmed: 8230445]
4 - The protein network of HIV budding
von Schwedler UK, Stuchell M, Muller B, Ward DM, Chung HY, Morita E, Wang HE, Davis T, He GP, Cimbora DM, Scott A, Krausslich HG, Kaplan J, Morham SG, Sundquist WI.
Cell 114(6): 701-713 (2003) [pubmed: 14505570]



Page last updated by Tulio de Oliveira.