HIVseq accepts user-submitted RT and protease sequences, compares them to the consensus subtype B reference sequence, and uses the differences as query parameters for interrogating the HIV Drug Resistance database. This allows users to detect unusual sequence results immediately so that the person doing the sequencing can check the primary sequence output while it is still on the desktop. In addition, unexpected associations between sequences or isolates can be discovered by immediately retrieving data on isolates sharing one or more mutations with the sequence.
On July 27, the program was updated to provide subtype-specific mutation prevalence for subtypes A, AE, AG, C, D, F, and G in addition to subtype B. The ability to extend the program was made possible by a multicenter collaborative effort of the Non-B HIV Drug Resistance Working Group
. The mutation prevalence data underlying HIVseq can be queried directly using the Mutation Prevalence According to Subtype and Treatment
A detailed description of the program as well as all updates can be found in the Release Notes.